4×lithium dodecyl sulfate lds (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher 4×lithium dodecyl sulfate lds
4×Lithium Dodecyl Sulfate Lds, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sample+buffer+%C2%B7+4/LDS-sample+buffer+(4X)%2C+reducing/pm42350042-73-4-10
Average 99 stars, based on 1 article reviews

4×lithium dodecyl sulfate lds - by Bioz Stars, 2026-10

99/100 stars

Images

Polyacrylamide Gel Electrophoresis:

Article Title: Chimeric switch scaffold protein augments CAR synapse formation and signaling networks.
Article Snippet: Protein concentrations were measured using the BCA assay (Thermo Fisher Scientific). .. Samples were mixed with 4×lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific), boiled at 70°C for 10 min, and separated on 4–12% BisTris sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS- PAGE) gels (Thermo Fisher Scientific). .. Proteins were transferred to polyvinylidene difluoride (PVDF) membranes using an iBlot2 Dry blotting system (Thermo Fisher Scientific).

SDS Page:

Concentration Assay:

Article Title: The presynaptic protein bassoon is a biofluid biomarker of synaptic pathology in multiple sclerosis
Article Snippet: .. The combined sample was dissolved in 10 mM ammonium bicarbonate (NH 4 HCO 3 ) to a final concentration of 1 μg μL −1 , and 50 μg of peptides were fractionated on a 25 cm ProSwiftTM RP-4H capillary monolithic column (Thermo Fisher Scientific) using an Agilent 1200 HPLC system. ..

High Performance Liquid Chromatography:

Laser Capture Microdissection:

Article Title: Elevated mitochondrial Ca 2+ impairs satellite cell pool expansion in response to skeletal muscle injury.
Article Snippet: .. PE-Cy7 rat anti-mouse CD31 clone 390 BD Biosciences 561410; RRID: AB_1061200 PE-Cy7 rat anti-mouse CD45 clone 30-F11 BD Biosciences 552848; RRID:A B_394489 Biotin rat anti-mouse CD106 clone 429 BD Biosciences 553331; RRID: AB_394787 PE Streptavidin BD Biosciences 554061; RRID: AB_10053328 α-7 integrin 647 clone R2F2 AbLab 67-0010-05; RRID: AB_2890939 MICU1 Sigma Aldrich HPA037480; RRID: AB_10696934 MCU Cell Signaling 14997S; RRID: AB_2721812 EMRE New England Peptide custom antibody VDAC1 Santa Cruz Biotechnology sc-390996; RRID: AB_2750920 IRDye 800CW anti-rabbit secondary LI-COR 926–32211; RRID: AB_621843 IRDye 680LT anti-mouse secondary LI-COR 926–68020; RRID: AB_10706161 HRP-conjugated anti-rabbit secondary Cell Signaling Technology 7074; RRID: AB_2099233 anti-Pax7 mouse IgG1 Developmental Studies Hybridoma Bank PAX7; RRID: AB_2299243 MYH1E myosin heavy chain Developmental Studies Hybridoma Bank MF20; RRID: AB_2147781 goat anti-mouse biotin-conjugated secondary antibody Jackson Immuno Research Laboratories Inc 115-065-205; RRID: AB_2338571 monoclonal antibody to laminin clone LAM-89 Sigma Aldrich L8271; RRID: AB_477162 Alexa Fluor 488 goat anti-mouse IgG antibody Life Technologies A11029; RRID: AB_2534088 Chemicals, peptides, and recombinant proteins tamoxifen diet Envigo TD.140251 tamoxifen Sigma T5648-1G cardiotoxin Sigma 217503 collagenase type II Gibco 17101–015 dispase Gibco 17105–041 RIPA buffer Thermo Scientific 89900 Halt Protease and Phosphatase Inhibitor Cocktail Thermo Scientific 78440 Bolt LDS sample buffer Invitrogen B0007 Bolt Bis-Tris 4–12% gels Invitrogen NW04120BOX TBS blocking buffer LI-COR 927–60001 Clarity Western ECL Substrate BioRad 1705060 oligomycin Sigma O4876 carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Sigma C2920 (Continued on next page) 10 Stem Cell Reports | Vol. .. PE-Cy7 rat anti-mouse CD31 clone 390 BD Biosciences 561410; RRID: AB_1061200 PE-Cy7 rat anti-mouse CD45 clone 30-F11 BD Biosciences 552848; RRID:A B_394489 Biotin rat anti-mouse CD106 clone 429 BD Biosciences 553331; RRID: AB_394787 PE Streptavidin BD Biosciences 554061; RRID: AB_10053328 α-7 integrin 647 clone R2F2 AbLab 67-0010-05; RRID: AB_2890939 MICU1 Sigma Aldrich HPA037480; RRID: AB_10696934 MCU Cell Signaling 14997S; RRID: AB_2721812 EMRE New England Peptide custom antibody VDAC1 Santa Cruz Biotechnology sc-390996; RRID: AB_2750920 IRDye 800CW anti-rabbit secondary LI-COR 926–32211; RRID: AB_621843 IRDye 680LT anti-mouse secondary LI-COR 926–68020; RRID: AB_10706161 HRP-conjugated anti-rabbit secondary Cell Signaling Technology 7074; RRID: AB_2099233 anti-Pax7 mouse IgG1 Developmental Studies Hybridoma Bank PAX7; RRID: AB_2299243 MYH1E myosin heavy chain Developmental Studies Hybridoma Bank MF20; RRID: AB_2147781 goat anti-mouse biotin-conjugated secondary antibody Jackson Immuno Research Laboratories Inc 115-065-205; RRID: AB_2338571 monoclonal antibody to laminin clone LAM-89 Sigma Aldrich L8271; RRID: AB_477162 Alexa Fluor 488 goat anti-mouse IgG antibody Life Technologies A11029; RRID: AB_2534088 Chemicals, peptides, and recombinant proteins tamoxifen diet Envigo TD.140251 tamoxifen Sigma T5648-1G cardiotoxin Sigma 217503 collagenase type II Gibco 17101–015 dispase Gibco 17105–041 RIPA buffer Thermo Scientific 89900 Halt Protease and Phosphatase Inhibitor Cocktail Thermo Scientific 78440 Bolt LDS sample buffer Invitrogen B0007 Bolt Bis-Tris 4–12% gels Invitrogen NW04120BOX TBS blocking buffer LI-COR 927–60001 Clarity Western ECL Substrate BioRad 1705060 oligomycin Sigma O4876 carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Sigma C2920 (Continued on next page) 10 Stem Cell Reports | Vol.

Recombinant:

Blocking Assay:

Western Blot:

Negative Control:

Article Title: Fc region variant
Article Snippet: .. A sample prepared by adding a phosphate buffer (pH 7.4) (Gibco) instead of the immune complex was used as the negative control. .. The produced plasmids were introduced transiently into the HEK293H cell line derived from human embryonic kidney cancer cells (Invitrogen) or into FreeStyle293 cells (Invitrogen) for antibody expression.

Protease Inhibitor:

Article Title: Immortalization of human airway epithelial cells for genome editing applications
Article Snippet: .. Differentiated cells were trypsinized and lysed in Laemmli buffer (120 mM Tris-HCl pH 6.8, 20 % glycerol, 4 % sodium dodecyl sulfate (SDS), protease inhibitor (Fisher Emergo B.V.)), and the lysates were homogenized using a QIAshredder (QIAGEN N.V., Germany) column to reduce viscosity. .. Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions.

Review